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IFN CXCL10, CCL5 and IL 15 expression was also upregulated by 18 , 21 and 2.5 fold, respectively, in USP18 silenced dispersed human pancreatic islets (Figure 3c). (a INS 1E cells (a), primary rat beta cells (b) and dispersed human islet cells (c) were transfected with siCTRL or siUSP18 and, after 48 of recovery, they casque beats pas cher were left untreated or treated with IFN for 24 (a) or 48 (b, c). CXCL10, CCL5 and IL 15 were assayed by RT PCR and normalized for the housekeeping genes GAPDH (a, b) or (c).
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